Heparinase treatment of heparin-contaminated plasma from coronary artery bypass grafting patients enables reliable quantification of microRNAs

BACKGROUND: microRNAs have recently been identified as powerful biomarkers of human disease. Reliable polymerase chain reaction (PCR)-based quantification of nucleic acids in clinical samples contaminated with polymerase inhibitor heparin requires deheparinization. However, the effects of deheparini...

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Detalhes bibliográficos
Publicado no:Biomol Detect Quantif
Main Authors: Kondratov, Kirill, Kurapeev, Dmitry, Popov, Maxim, Sidorova, Marina, Minasian, Sarkis, Galagudza, Michael, Kostareva, Anna, Fedorov, Anton
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2016
Assuntos:
Research Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4906134/
https://ncbi.nlm.nih.gov/pubmed/27335806
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bdq.2016.03.001
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