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Conventional fluorescence microscopy below the diffraction limit with simultaneous capture of two fluorophores in DNA origami

A conventional fluorescence microscope was previously constructed for simultaneous imaging of two colors to gain subdiffraction localization. The system is predicated on color separation of overlapping Airy discs, construction of matrices of Cartesian coordinates to determine locations as well as ce...

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Detalhes bibliográficos
Publicado no:Proc SPIE Int Soc Opt Eng
Autor principal: Glasgow, Ben J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4905728/
https://ncbi.nlm.nih.gov/pubmed/27307653
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/12.2211074
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