PCR Strategies for Complete Allele Calling in Multigene Families Using High-Throughput Sequencing Approaches

The characterization of multigene families with high copy number variation is often approached through PCR amplification with highly degenerate primers to account for all expected variants flanking the region of interest. Such an approach often introduces PCR biases that result in an unbalanced repr...

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Udgivet i:PLoS One
Main Authors: Marmesat, Elena, Soriano, Laura, Mazzoni, Camila J., Sommer, Simone, Godoy, José A.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2016
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4905633/
https://ncbi.nlm.nih.gov/pubmed/27294261
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0157402
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