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Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions
This article reviews current methods for the reliable heterologous overexpression in Escherichia coli and purification of milligram quantities of bacterial membrane sensor kinase (MSK) proteins belonging to the two-component signal transduction family of integral membrane proteins. Many of these met...
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| 發表在: | Biochem Soc Trans |
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| Main Authors: | , , , , , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Portland Press Ltd.
2016
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4900758/ https://ncbi.nlm.nih.gov/pubmed/27284046 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BST20160023 |
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