Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions

This article reviews current methods for the reliable heterologous overexpression in Escherichia coli and purification of milligram quantities of bacterial membrane sensor kinase (MSK) proteins belonging to the two-component signal transduction family of integral membrane proteins. Many of these met...

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Detalhes bibliográficos
Publicado no:Biochem Soc Trans
Main Authors: Hussain, Rohanah, Harding, Stephen E., Hughes, Charlotte S., Ma, Pikyee, Patching, Simon G., Edara, Shalini, Siligardi, Giuliano, Henderson, Peter J.F., Phillips-Jones, Mary K.
Formato: Artigo
Idioma:Inglês
Publicado em: Portland Press Ltd. 2016
Assuntos:
Biochemical Society Focused Meetings
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4900758/
https://ncbi.nlm.nih.gov/pubmed/27284046
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BST20160023
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