Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions

This article reviews current methods for the reliable heterologous overexpression in Escherichia coli and purification of milligram quantities of bacterial membrane sensor kinase (MSK) proteins belonging to the two-component signal transduction family of integral membrane proteins. Many of these met...

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Foilsithe in:Biochem Soc Trans
Main Authors: Hussain, Rohanah, Harding, Stephen E., Hughes, Charlotte S., Ma, Pikyee, Patching, Simon G., Edara, Shalini, Siligardi, Giuliano, Henderson, Peter J.F., Phillips-Jones, Mary K.
Formáid: Artigo
Teanga:Inglês
Foilsithe: Portland Press Ltd. 2016
Ábhair:
Biochemical Society Focused Meetings
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4900758/
https://ncbi.nlm.nih.gov/pubmed/27284046
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BST20160023
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