Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions

This article reviews current methods for the reliable heterologous overexpression in Escherichia coli and purification of milligram quantities of bacterial membrane sensor kinase (MSK) proteins belonging to the two-component signal transduction family of integral membrane proteins. Many of these met...

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Udgivet i:Biochem Soc Trans
Main Authors: Hussain, Rohanah, Harding, Stephen E., Hughes, Charlotte S., Ma, Pikyee, Patching, Simon G., Edara, Shalini, Siligardi, Giuliano, Henderson, Peter J.F., Phillips-Jones, Mary K.
Format: Artigo
Sprog:Inglês
Udgivet: Portland Press Ltd. 2016
Fag:
Biochemical Society Focused Meetings
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4900758/
https://ncbi.nlm.nih.gov/pubmed/27284046
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BST20160023
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