Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions

This article reviews current methods for the reliable heterologous overexpression in Escherichia coli and purification of milligram quantities of bacterial membrane sensor kinase (MSK) proteins belonging to the two-component signal transduction family of integral membrane proteins. Many of these met...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Biochem Soc Trans
Prif Awduron: Hussain, Rohanah, Harding, Stephen E., Hughes, Charlotte S., Ma, Pikyee, Patching, Simon G., Edara, Shalini, Siligardi, Giuliano, Henderson, Peter J.F., Phillips-Jones, Mary K.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Portland Press Ltd. 2016
Pynciau:
Biochemical Society Focused Meetings
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4900758/
https://ncbi.nlm.nih.gov/pubmed/27284046
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BST20160023
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