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A method for sectioning and immunohistochemical analysis of stem cell-derived 3D organoids
This unit describes a protocol for embedding, sectioning and immunocytochemical analysis of pluripotent stem cell-derived 3D organoids. Specifically, we describe a method to embed iPSC-derived retinal cups in low-melt agarose, acquire thick sections using a vibratome tissue slicer, and perform immun...
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| Publicado no: | Curr Protoc Stem Cell Biol |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4900235/ https://ncbi.nlm.nih.gov/pubmed/27171793 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpsc.3 |
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