Characterising the Canine Oral Microbiome by Direct Sequencing of Reverse-Transcribed rRNA Molecules

PCR amplification and sequencing of phylogenetic markers, primarily Small Sub-Unit ribosomal RNA (SSU rRNA) genes, has been the paradigm for defining the taxonomic composition of microbiomes. However, ‘universal’ SSU rRNA gene PCR primer sets are likely to miss much of the diversity therein. We sequ...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: McDonald, James E., Larsen, Niels, Pennington, Andrea, Connolly, John, Wallis, Corrin, Rooks, David J., Hall, Neil, McCarthy, Alan J., Allison, Heather E.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4898712/
https://ncbi.nlm.nih.gov/pubmed/27276347
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0157046
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