Effect of cryopreservation of peripheral blood mononuclear cells (PBMCs) on the variability of an antigen-specific memory B cell ELISpot

The ELISpot assay is used in vaccine studies for the quantification of antigen-specific memory B cells (B(MEM)), and can be performed using cryopreserved samples. The effects of cryopreservation on B(MEM) detection and the consistency of cultured ELISpot assays when performed by different operators...

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Detalhes bibliográficos
Publicado no:Hum Vaccin Immunother
Main Authors: Trück, Johannes, Mitchell, Ruth, Thompson, Amber J, Morales-Aza, Begonia, Clutterbuck, Elizabeth A, Kelly, Dominic F, Finn, Adam, Pollard, Andrew J
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 2014
Assuntos:
Research Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4896800/
https://ncbi.nlm.nih.gov/pubmed/25424961
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/hv.29318
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