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Single-molecule FRET states, conformational interchange, and conformational selection by dye labels in calmodulin

We investigate the roles of measurement time scale and the nature of the fluorophores in the FRET states measured for calmodulin, a calcium signaling protein known to undergo pronounced conformational changes. The measured FRET distributions depend markedly on the measurement time scale (nanosecond...

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Publicado en:J Phys Chem B
Autores principales: DeVore, Matthew S., Braimah, Adebayo, Benson, David R., Johnson, Carey K.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2016
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4894822/
https://ncbi.nlm.nih.gov/pubmed/27111039
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jpcb.6b00864
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