Toward a more accurate view of human B-cell repertoire by next-generation sequencing, unbiased repertoire capture and single-molecule barcoding

B-cell repertoire analysis using next-generation sequencing has become a valuable tool for interrogating the genetic record of humoral response to infection. However, key obstacles such as low throughput, short read length, high error rate, and undetermined bias of multiplex PCR method have hindered...

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Publicado en:Sci Rep
Autores principales: He, Linling, Sok, Devin, Azadnia, Parisa, Hsueh, Jessica, Landais, Elise, Simek, Melissa, Koff, Wayne C., Poignard, Pascal, Burton, Dennis R., Zhu, Jiang
Formato: Artigo
Lenguaje:Inglês
Publicado: Nature Publishing Group 2014
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4894419/
https://ncbi.nlm.nih.gov/pubmed/25345460
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep06778
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