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The source of SYBR green master mix determines outcome of nucleic acid amplification reactions

BACKGROUND: Quantitative (q) PCR by amplification of nucleic acid with a fluorescent dye is widely used. Selection of adequate PCR reagents and devices is relevant to achieve reliable and consistent data. Our main objective was to test the robustness of different commercial SYBR green PCR mixes with...

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Detalhes bibliográficos
Publicado no:BMC Res Notes
Main Authors: Yang, Jianxin, Kemps-Mols, Berit, Spruyt-Gerritse, Marijke, Anholts, Jacqueline, Claas, Frans, Eikmans, Michael
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2016
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4893258/
https://ncbi.nlm.nih.gov/pubmed/27259280
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13104-016-2093-4
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