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Detection of RNA-DNA association by a proximity ligation-based method
We describe a proximity ligation assay (PLA)-based method of assessing association of DNA and RNA in single cells during the cell cycle. Pulse-labeling of DNA with EdU and RNA with BrU and testing their close proximity by PLA demonstrates that RNA synthesis in individual cells resumes about 30–45 mi...
Enregistré dans:
| Publié dans: | Sci Rep |
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| Auteurs principaux: | , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Nature Publishing Group
2016
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4891662/ https://ncbi.nlm.nih.gov/pubmed/27256324 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep27313 |
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