Concentric-Flow Electrokinetic Injector Enables Serial Crystallography of Ribosome and Photosystem-II

In this work, a concentric-flow electrokinetic injector delivered microcrystals of Geobacillus stearothermophilus thermolysin (2.2 Å structure), Thermosynechococcus elongatus photosystem II (< 3 Å diffraction) and Thermus thermophilus small ribosomal subunit (3.4 Å structure). The first ambient-t...

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Publié dans:Nat Methods
Auteurs principaux: Sierra, Raymond G., Gati, Cornelius, Laksmono, Hartawan, Dao, E. Han, Gul, Sheraz, Fuller, Franklin, Kern, Jan, Chatterjee, Ruchira, Ibrahim, Mohamed, Brewster, Aaron S., Young, Iris D., Michels-Clark, Tara, Aquila, Andrew, Liang, Mengning, Hunter, Mark S., Koglin, Jason E., Boutet, Sébastien, Junco, Elia A., Hayes, Brandon, Bogan, Michael J., Hampton, Christina Y., Puglisi, Elisabetta V., Sauter, Nicholas K., Stan, Claudiu A., Zouni, Athina, Yano, Junko, Yachandra, Vittal K., Soltis, S. Michael, Puglisi, Joseph D., DeMirci, Hasan
Format: Artigo
Langue:Inglês
Publié: 2015
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4890631/
https://ncbi.nlm.nih.gov/pubmed/26619013
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3667
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