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SOD2 targeted gene editing by CRISPR/Cas9 yields human cells devoid of MnSOD

To date no models exist to study MnSOD deficiency in human cells. To address this deficiency, we created a SOD2-null human cell line that is completely devoid of detectable MnSOD protein expression and enzyme activity. We utilized the CRISPR/Cas9 system to generate biallelic SOD2 disruption in HEK29...

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Foilsithe in:Free Radic Biol Med
Main Authors: Cramer-Morales, Kimberly, Heer, Collin, Mapuskar, Kranti, Domann, Frederick E.
Formáid: Artigo
Teanga:Inglês
Foilsithe: 2015
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4890619/
https://ncbi.nlm.nih.gov/pubmed/26208779
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.freeradbiomed.2015.07.017
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