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Long-range transcriptional interference in E. coli used to construct a dual positive selection system for genetic switches
We have investigated transcriptional interference between convergent genes in E. coli and demonstrate substantial interference for inter-promoter distances of as far as 3 kb. Interference can be elicited by both strong σ(70) dependent and T7 promoters. In the presented design, a strong promoter driv...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
2016
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4889929/ https://ncbi.nlm.nih.gov/pubmed/26932362 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw125 |
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