Subnuclear localization, rates and effectiveness of UVC-induced unscheduled DNA synthesis visualized by fluorescence widefield, confocal and super-resolution microscopy

Unscheduled DNA synthesis (UDS) is the final stage of the process of repair of DNA lesions induced by UVC. We detected UDS using a DNA precursor, 5-ethynyl-2′-deoxyuridine (EdU). Using wide-field, confocal and super-resolution fluorescence microscopy and normal human fibroblasts, derived from health...

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Publicado en:Cell Cycle
Main Authors: Pierzyńska-Mach, Agnieszka, Szczurek, Aleksander, Cella Zanacchi, Francesca, Pennacchietti, Francesca, Drukała, Justyna, Diaspro, Alberto, Cremer, Christoph, Darzynkiewicz, Zbigniew, Dobrucki, Jurek W.
Formato: Artigo
Idioma:Inglês
Publicado: Taylor & Francis 2016
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4889244/
https://ncbi.nlm.nih.gov/pubmed/27097376
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15384101.2016.1158377
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