Replicates, Read Numbers, and Other Important Experimental Design Considerations for Microbial RNA-seq Identified Using Bacillus thuringiensis Datasets

RNA-seq is being used increasingly for gene expression studies and it is revolutionizing the fields of genomics and transcriptomics. However, the field of RNA-seq analysis is still evolving. Therefore, we specifically designed this study to contain large numbers of reads and four biological replicat...

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Detalhes bibliográficos
Publicado no:Front Microbiol
Main Authors: Manga, Punita, Klingeman, Dawn M., Lu, Tse-Yuan S., Mehlhorn, Tonia L., Pelletier, Dale A., Hauser, Loren J., Wilson, Charlotte M., Brown, Steven D.
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2016
Assuntos:
Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4886094/
https://ncbi.nlm.nih.gov/pubmed/27303383
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2016.00794
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