Improvement of Mitochondria Extract from Saccharomyces cerevisiae Characterization in Shotgun Proteomics Using Sheathless Capillary Electrophoresis Coupled to Tandem Mass Spectrometry

In this work, we describe the characterization of a quantity-limited sample (100 ng) of yeast mitochondria by shotgun bottom-up proteomics. Sample characterization was carried out by sheathless capillary electrophoresis, equipped with a high sensitivity porous tip and coupled to tandem mass spectrom...

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Detalhes bibliográficos
Publicado no:J Chromatogr Sci
Main Authors: Ibrahim, Marianne, Gahoual, Rabah, Enkler, Ludovic, Becker, Hubert Dominique, Chicher, Johana, Hammann, Philippe, François, Yannis-Nicolas, Kuhn, Lauriane, Leize-Wagner, Emmanuelle
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2016
Assuntos:
Electrophoresis
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4885408/
https://ncbi.nlm.nih.gov/pubmed/26860395
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/chromsci/bmw005
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