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Nanomechanical and Thermophoretic Analyses of the Nucleotide-Dependent Interactions between the AAA(+) Subunits of Magnesium Chelatase

[Image: see text] In chlorophyll biosynthesis, the magnesium chelatase enzyme complex catalyzes the insertion of a Mg(2+) ion into protoporphyrin IX. Prior to this event, two of the three subunits, the AAA(+) proteins ChlI and ChlD, form a ChlID–MgATP complex. We used microscale thermophoresis to di...

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Pubblicato in:J Am Chem Soc
Autori principali: Adams, Nathan B. P., Vasilev, Cvetelin, Brindley, Amanda A., Hunter, C. Neil
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Chemical Society 2016
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4882731/
https://ncbi.nlm.nih.gov/pubmed/27133226
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.6b02827
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