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A simple and rapid method for combining fluorescent in situ RNA hybridization (FISH) and immunofluorescence in the C. elegans germline

Imaging of RNAs and proteins in specific tissues has opened ample avenues to understand gene expression during development. Recently, a fluorescent in situ RNA hybridization (FISH) method has been developed to analyze the spatio-temporal expression patterns of endogenous mRNAs. However, combining FI...

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Detalhes bibliográficos
Publicado no:MethodsX
Main Authors: Yoon, Dong Suk, Pendergrass, DeQwon L., Lee, Myon-Hee
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4878318/
https://ncbi.nlm.nih.gov/pubmed/27257608
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2016.05.001
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