Photoaffinity labeling of the primary fibrin polymerization site: localization of the label to gamma-chain Tyr-363.
Fragment D prepared from human fibrinogen was labeled specifically by photoactivation of the peptide [14C]Gly-Pro-Arg-N-(4-azido-2-nitrophenyl)Lys amide. The preparation was freed of excess labeling reagents and then reduced and alkylated. The component alpha, beta, and gamma chains were purified by...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1992
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC48769/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1557396/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.7.2893 |
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