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Photoaffinity labeling of the primary fibrin polymerization site: localization of the label to gamma-chain Tyr-363.

Fragment D prepared from human fibrinogen was labeled specifically by photoactivation of the peptide [14C]Gly-Pro-Arg-N-(4-azido-2-nitrophenyl)Lys amide. The preparation was freed of excess labeling reagents and then reduced and alkylated. The component alpha, beta, and gamma chains were purified by...

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Détails bibliographiques
Publié dans:Proc Natl Acad Sci U S A
Auteurs principaux: Yamazumi, K, Doolittle, R F
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 1992
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC48769/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1557396/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.7.2893
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