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Photoaffinity labeling of the primary fibrin polymerization site: localization of the label to gamma-chain Tyr-363.

Fragment D prepared from human fibrinogen was labeled specifically by photoactivation of the peptide [14C]Gly-Pro-Arg-N-(4-azido-2-nitrophenyl)Lys amide. The preparation was freed of excess labeling reagents and then reduced and alkylated. The component alpha, beta, and gamma chains were purified by...

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Detalhes bibliográficos
Main Authors: Yamazumi, K, Doolittle, R F
Formato: Artigo
Idioma:Inglês
Publicado em: 1992
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC48769/
https://ncbi.nlm.nih.gov/pubmed/1557396
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