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A simplified method for reconstituting active E. coli DNA polymerase III
Genome duplication in E. coli is carried out by DNA polymerase III, an enzyme complex consisting of ten subunits. Investigations of the biochemical and structural properties of DNA polymerase III require the expression and purification of subunits including α, ge, θ, γ, δ′, δ, and β separately follo...
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| Publicado no: | Protein Cell |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Higher Education Press
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4875203/ https://ncbi.nlm.nih.gov/pubmed/21499845 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13238-011-1032-3 |
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