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Illumina MiSeq sequencing disfavours a sequence motif in the GFP reporter gene
Green fluorescent protein (GFP) is one of the most used reporter genes. We have used next-generation sequencing (NGS) to analyse the genetic diversity of a recombinant influenza A virus that expresses GFP and found a remarkable coverage dip in the GFP coding sequence. This coverage dip was present w...
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| 出版年: | Sci Rep |
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| 主要な著者: | , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Nature Publishing Group
2016
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4872057/ https://ncbi.nlm.nih.gov/pubmed/27193250 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep26314 |
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