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Nitrogen-detected TROSY yields comparable sensitivity to proton-detected TROSY for non-deuterated, large proteins under physiological salt conditions

Direct detection of the TROSY component of proton-attached (15)N nuclei ((15)N-detected TROSY) yields high quality spectra with high field magnets, by taking advantage of the slow (15)N transverse relaxation. The slow transverse relaxation and narrow line width of the (15)N-detected TROSY resonances...

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Bibliografski detalji
Izdano u:J Biomol NMR
Glavni autori: Takeuchi, Koh, Arthanari, Haribabu, Imai, Misaki, Wagner, Gerhard, Shimada, Ichio
Format: Artigo
Jezik:Inglês
Izdano: 2016
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4871712/
https://ncbi.nlm.nih.gov/pubmed/26800993
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-016-0015-3
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