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Measuring bioenergetics in T cells using a Seahorse Extracellular Flux Analyzer
This unit contains several protocols to determine the energy utilization of T cells in real-time using a Seahorse Extracellular Flux Analyzer (www.seahorsebio.com). The advantages to using this machine over traditional metabolic assays include the simultaneous measurement of glycolysis and mitochond...
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| Udgivet i: | Curr Protoc Immunol |
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| Main Authors: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2016
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4864360/ https://ncbi.nlm.nih.gov/pubmed/27038461 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471142735.im0316bs113 |
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