Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2

This protocol describes a method to obtain spatially resolved proteomic maps of specific compartments within living mammalian cells. An engineered peroxidase, APEX2, is genetically targeted to a cellular region of interest. Upon the addition of hydrogen peroxide for 1 min to cells preloaded with a b...

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Bibliografiske detaljer
Udgivet i:Nat Protoc
Main Authors: Hung, Victoria, Udeshi, Namrata D, Lam, Stephanie S, Loh, Ken H, Cox, Kurt J, Pedram, Kayvon, Carr, Steven A, Ting, Alice Y
Format: Artigo
Sprog:Inglês
Udgivet: 2016
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4863649/
https://ncbi.nlm.nih.gov/pubmed/26866790
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.018
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