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Use of Protein G Microcolumns in Chromatographic Immunoassays: A Comparison of Competitive Binding Formats

Affinity microcolumns containing protein G were used as general platforms for creating chromatographic-based competitive binding immunoassays. Human serum albumin (HSA) was used as a model target for this work and HSA tagged with a near infrared fluorescent dye was utilized as the label. The protein...

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Bibliographische Detailangaben
Veröffentlicht in:J Chromatogr B Analyt Technol Biomed Life Sci
Hauptverfasser: Pfaunmiller, Erika L., Anguizola, Jeanethe A., Milanuk, Mitchell L., Carter, NaTasha, Hage, David S.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2016
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4862902/
https://ncbi.nlm.nih.gov/pubmed/26777776
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jchromb.2015.12.055
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