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Improvements to the HITS-CLIP protocol eliminate widespread mispriming artifacts

BACKGROUND:  High-throughput sequencing of RNA isolated by crosslinking immunoprecipitation (HITS-CLIP) allows for high resolution, genome-wide mapping of RNA-binding proteins. This methodology is frequently used to validate predicted targets of microRNA binding, as well as direct targets of other R...

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Publicat a:BMC Genomics
Autors principals: Gillen, Austin E., Yamamoto, Tomomi M., Kline, Enos, Hesselberth, Jay R., Kabos, Peter
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2016
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4858895/
https://ncbi.nlm.nih.gov/pubmed/27150721
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-016-2675-5
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