Two RNAs or DNAs May Artificially Fuse Together at a Short Homologous Sequence (SHS) during Reverse Transcription or Polymerase Chain Reactions, and Thus Reporting an SHS-Containing Chimeric RNA Requires Extra Caution

Tens of thousands of chimeric RNAs have been reported. Most of them contain a short homologous sequence (SHS) at the joining site of the two partner genes but are not associated with a fusion gene. We hypothesize that many of these chimeras may be technical artifacts derived from SHS-caused mis-prim...

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Bibliographic Details
Published in:PLoS One
Main Authors: Xie, Bingkun, Yang, Wei, Ouyang, Yongchang, Chen, Lichan, Jiang, Hesheng, Liao, Yuying, Liao, D. Joshua
Format: Artigo
Language:Inglês
Published: Public Library of Science 2016
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4858267/
https://ncbi.nlm.nih.gov/pubmed/27148738
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0154855
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