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Quantitative Real-Time Polymerase Chain Reaction Measurement of HLA-DRA Gene Expression in Whole Blood Is Highly Reproducible and Shows Changes That Reflect Dynamic Shifts in Monocyte Surface HLA-DR Expression during the Course of Sepsis

INTRODUCTION: A decrease in the expression of monocyte surface protein HLA-DR (mHLA-DR), measured by flow cytometry (FCM), has been suggested as a marker of immunosuppression and negative outcome in severe sepsis. However, FCM is not always available due to sample preparation that limits its use to...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Cajander, Sara, Tina, Elisabet, Bäckman, Anders, Magnuson, Anders, Strålin, Kristoffer, Söderquist, Bo, Källman, Jan
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4856385/
https://ncbi.nlm.nih.gov/pubmed/27144640
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0154690
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