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Enhanced snoMEN Vectors Facilitate Establishment of GFP–HIF-1α Protein Replacement Human Cell Lines

The snoMEN (snoRNA Modulator of gene ExpressioN) vector technology was developed from a human box C/D snoRNA, HBII-180C, which contains an internal sequence that can be manipulated to make it complementary to RNA targets, allowing knock-down of targeted genes. Here we have screened additional human...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Ono, Motoharu, Yamada, Kayo, Bensaddek, Dalila, Afzal, Vackar, Biddlestone, John, Ortmann, Brian, Mudie, Sharon, Boivin, Vincent, Scott, Michelle S., Rocha, Sonia, Lamond, Angus I.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4851398/
https://ncbi.nlm.nih.gov/pubmed/27128805
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0154759
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