First principles design of a core bioenergetic transmembrane electron transfer protein

Here we describe the design, E. coli expression and characterization of a simplified, adaptable and functionally transparent single chain 4-α-helix transmembrane protein frame that binds multiple heme and light activatable porphyrins. Such man-made cofactor-binding oxidoreductases, designed from fir...

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Bibliografiske detaljer
Udgivet i:	Biochim Biophys Acta
Main Authors:	Goparaju, Geetha, Fry, Bryan A., Chobot, Sarah E., Wiedman, Gregory, Moser, Christopher C., Dutton, P. Leslie, Discher, Bohdana M.
Format:	Artigo
Sprog:	Inglês
Udgivet:	2015
Fag:	Article
Online adgang:	https://ncbi.nlm.nih.gov/pmc/articles/PMC4846532/ https://ncbi.nlm.nih.gov/pubmed/26672896 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbabio.2015.12.002
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First principles design of a core bioenergetic transmembrane electron transfer protein

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