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Axial superresolution via multiangle TIRF microscopy with sequential imaging and photobleaching

We report superresolution optical sectioning using a multiangle total internal reflection fluorescence (TIRF) microscope. TIRF images were constructed from several layers within a normal TIRF excitation zone by sequentially imaging and photobleaching the fluorescent molecules. The depth of the evane...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Fu, Yan, Winter, Peter W., Rojas, Raul, Wang, Victor, McAuliffe, Matthew, Patterson, George H.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4843470/
https://ncbi.nlm.nih.gov/pubmed/27044072
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1516715113
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