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A CRISPR/Cas9 system adapted for gene editing in marine algae

Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen...

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Dettagli Bibliografici
Pubblicato in:Sci Rep
Autori principali: Nymark, Marianne, Sharma, Amit Kumar, Sparstad, Torfinn, Bones, Atle M., Winge, Per
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group 2016
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4842962/
https://ncbi.nlm.nih.gov/pubmed/27108533
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep24951
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