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Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

The vast majority of mass spectrometry (MS)-based protein analysis methods involve an enzymatic digestion step prior to detection, typically with trypsin. This step is necessary for the generation of small molecular weight peptides, generally with MW < 3,000-4,000 Da, that fall within the effecti...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Lazar, Iulia M., Deng, Jingren, Smith, Nicole
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4841366/
https://ncbi.nlm.nih.gov/pubmed/27078683
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53564
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