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Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
The vast majority of mass spectrometry (MS)-based protein analysis methods involve an enzymatic digestion step prior to detection, typically with trypsin. This step is necessary for the generation of small molecular weight peptides, generally with MW < 3,000-4,000 Da, that fall within the effecti...
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Publicado no: | J Vis Exp |
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Main Authors: | , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
MyJove Corporation
2016
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4841366/ https://ncbi.nlm.nih.gov/pubmed/27078683 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53564 |
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