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Quantitative Microplate Assay for Real-Time Nuclease Kinetics
Utilizing the phenomenon of nucleases exposing oligonucleotide phosphate backbones to phosphatases we present a novel quantitative method for kinetics of nuclease catalysis. Inorganic phosphate released from nuclease products by phosphatases could be quantified in real-time by a fluorescent sensor o...
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| Publicado no: | PLoS One |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4839650/ https://ncbi.nlm.nih.gov/pubmed/27101307 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0154099 |
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