Germline Gene Editing in Chickens by Efficient CRISPR-Mediated Homologous Recombination in Primordial Germ Cells

The CRISPR/Cas9 system has been applied in a large number of animal and plant species for genome editing. In chickens, CRISPR has been used to knockout genes in somatic tissues, but no CRISPR-mediated germline modification has yet been reported. Here we use CRISPR to target the chicken immunoglobuli...

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Détails bibliographiques
Publié dans:PLoS One
Auteurs principaux: Dimitrov, Lazar, Pedersen, Darlene, Ching, Kathryn H., Yi, Henry, Collarini, Ellen J., Izquierdo, Shelley, van de Lavoir, Marie-Cecile, Leighton, Philip A.
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2016
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4839619/
https://ncbi.nlm.nih.gov/pubmed/27099923
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0154303
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