Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to “MS2 coat proteins bound to yeast mRNAs block 5′ to 3′ degradation and trap mRNA decay products: implications for the localization of mRNAs by MS2-MCP system”

The MS2 system has been extensively used to visualize single mRNA molecules in live cells and follow their localization and behavior. In their Letter to the Editor recently published, Garcia and Parker suggest that use of the MS2 system may yield erroneous mRNA localization results due to the accumu...

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Detalhes bibliográficos
Publicado no:RNA
Main Authors: Haimovich, Gal, Zabezhinsky, Dmitry, Haas, Brian, Slobodin, Boris, Purushothaman, Pravinkumar, Fan, Lin, Levin, Joshua Z., Nusbaum, Chad, Gerst, Jeffrey E.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2016
Assuntos:
Letter to the Editor
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4836641/
https://ncbi.nlm.nih.gov/pubmed/26968626
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.055095.115
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