Estimates of Soil Bacterial Ribosome Content and Diversity Are Significantly Affected by the Nucleic Acid Extraction Method Employed

Modern sequencing technologies allow high-resolution analyses of total and potentially active soil microbial communities based on their DNA and RNA, respectively. In the present study, quantitative PCR and 454 pyrosequencing were used to evaluate the effects of different extraction methods on the ab...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Wüst, Pia K., Nacke, Heiko, Kaiser, Kristin, Marhan, Sven, Sikorski, Johannes, Kandeler, Ellen, Daniel, Rolf, Overmann, Jörg
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2016
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4836429/
https://ncbi.nlm.nih.gov/pubmed/26896137
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00019-16
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