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Rapid Purification and Characterization of Mutant Origin Recognition Complexes in Saccharomyces cerevisiae

Purification of the origin recognition complex (ORC) from wild-type budding yeast cells more than two decades ago opened up doors to analyze the initiation of eukaryotic chromosomal DNA replication biochemically. Although revised methods to purify ORC from overproducing cells were reported later, pu...

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Détails bibliographiques
Publié dans:Front Microbiol
Auteurs principaux: Kawakami, Hironori, Ohashi, Eiji, Tsurimoto, Toshiki, Katayama, Tsutomu
Format: Artigo
Langue:Inglês
Publié: Frontiers Media S.A. 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4834435/
https://ncbi.nlm.nih.gov/pubmed/27148210
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2016.00521
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