Identification of novel proteins binding the AU-rich element of α-prothymosin mRNA through the selection of open reading frames (RIDome)

We describe here a platform for high-throughput protein expression and interaction analysis aimed at identifying the RNA-interacting domainome. This approach combines the selection of a phage library displaying “filtered” open reading frames with next-generation DNA sequencing. The method was valida...

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Bibliografiske detaljer
Udgivet i:RNA Biol
Main Authors: Patrucco, Laura, Peano, Clelia, Chiesa, Andrea, Guida, Filomena, Luisi, Imma, Boria, Ilenia, Mignone, Flavio, De Bellis, Gianluca, Zucchelli, Silvia, Gustincich, Stefano, Santoro, Claudio, Sblattero, Daniele, Cotella, Diego
Format: Artigo
Sprog:Inglês
Udgivet: Taylor & Francis 2015
Fag:
Technical Paper
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4829324/
https://ncbi.nlm.nih.gov/pubmed/26512911
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15476286.2015.1107702
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