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Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
Retroviruses exhibit signature integration preferences on both the local and global scales. Here, we present a detailed protocol for (1) generation of diverse libraries of retroviral integration sites using ligation-mediated PCR (LM-PCR) amplification and next-generation sequencing (NGS), (2) mappin...
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| Publié dans: | J Vis Exp |
|---|---|
| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
MyJove Corporation
2016
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4829050/ https://ncbi.nlm.nih.gov/pubmed/27023428 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53840 |
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