Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

The protocol describes a quantitative RT-PCR method for the detection and quantification of EHV-2 in equine respiratory fluids according to the NF U47-600 norm. After the development and first validation step, two distinct characterization steps were performed according to the AFNOR norm: (a) charac...

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Bibliografiska uppgifter
I publikationen:J Vis Exp
Huvudupphovsmän: Hue, Erika S., Fortier, Christine I., Laurent, Aurélie M., Quesnelle, Yann F., Fortier, Guillaume D., Legrand, Loïc J., Pronost, Stephane L.
Materialtyp: Artigo
Språk:Inglês
Publicerad: MyJove Corporation 2016
Ämnen:
Immunology
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4829031/
https://ncbi.nlm.nih.gov/pubmed/27022998
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53672
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