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Three-dimensional Alginate-bead Culture of Human Pituitary Adenoma Cells
A three-dimensional culture method is described in which primary pituitary adenoma cells are grown in alginate beads. Alginate is a polymer derived from brown sea algae. Briefly, the tumor tissue is cut into small pieces and submitted to an enzymatic digestion with collagenase and trypsin. Next, a c...
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| Published in: | J Vis Exp |
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| Main Authors: | , , , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
MyJove Corporation
2016
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4828172/ https://ncbi.nlm.nih.gov/pubmed/26966916 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53637 |
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