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Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
This protocol presents a method to perform quantitative, single-cell in situ analyses of protein expression to study lineage specificationin mouse preimplantation embryos. The procedures necessary for embryo collection, immunofluorescence, imaging on a confocal microscope, and image segmentation and...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4828170/ https://ncbi.nlm.nih.gov/pubmed/26967230 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53654 |
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