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Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy
Freeze-fracture/freeze-etch describes a process whereby specimens, typically biological or nanomaterial in nature, are frozen, fractured, and replicated to generate a carbon/platinum “cast” intended for examination by transmission electron microscopy. Specimens are subjected to ultrarapid freezing r...
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| Publicado no: | J Vis Exp |
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| Autor principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4828069/ https://ncbi.nlm.nih.gov/pubmed/25285532 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51694 |
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