Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration

Previously described mitochondrial isolation methods using differential centrifugation and/or Ficoll gradient centrifugation require 60 to 100 min to complete. We describe a method for the rapid isolation of mitochondria from mammalian biopsies using a commercial tissue dissociator and differential...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:J Vis Exp
Main Authors: Preble, Janine M., Pacak, Christina A., Kondo, Hiroshi, MacKay, Allison A., Cowan, Douglas B., McCully, James D.
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2014
Teme:
Cellular Biology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4828055/
https://ncbi.nlm.nih.gov/pubmed/25225817
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51682
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